Sample requirements are detailed in the test-specific information; however, the following general guidelines apply.
Test |
Specimen Requirements |
| Molecular (DNA) tests | Adults: 4 ml in EDTA (purple top) Children: 1-2 ml in EDTA (purple top) For newborns and very young babies supply 1 ml EDTA (purple top) |
| Cytogenetics routine blood | Paediatric (0-6mths): 1-2 ml whole blood in a lithium heparin (LH) tube (green top) >6mths: 2-5 ml whole blood in a LH tube |
| Microarray | Children: 2-5 ml in both lithium heparin (green top) & EDTA (purple top) For newborns and very young babies supply 1-2 ml in both lithium heparin (green top) & EDTA (purple top) |
NGS Cancer Hotspot Panel |
NGS testing can be performed on bone marrow, leukaemic blood and FFPE samples Bone Marrow: 1-10ml in EDTA FFPE sample: Please provide 2-3 slides (minimum) cut at 4 microns along with an H&E slide with the area of interest circled |
| Chromosome breakage disorder | At least 6 ml in lithium heparin (green top) |
| Oncology Bone marrow / Leukaemic blood Tumour / Lymph node biopsy |
2-10 ml in lithium heparin (green top) Sample transported in sterile tissue culture media |
| Prenatal Amniotic fluid CVS Fetal blood |
10-20 ml 10-30 mg clean villi 1-2 ml in lithium heparin |
| Tissue | Unfixed sample transported in sterile saline solution |
| Non-invasive Prenatal Test | 8ml blood in streck tube (khaki) |
| FISH | Routine blood: Paediatric (0-6mths): 1-2 ml whole blood in a lithium heparin (LH) tube (green top) >6mths: 2-5 ml whole blood in a LH tube Bone Marrow / Leukaemic blood: 2-10 ml in lithium heparin (green top) Amniotic Fluid: 10-20 ml CVS: 10-30 mg clean villi Fetal blood: 1-2 ml in lithium heparin Paraffin Embedded Tissues (PETs): 6 x 2-4 µm sections are required for FISH analysis. A H&E slide should accompany the sections with the region of interest indicated. |
ABOUT USWellington Regional Genetics Laboratory (WRGL) provides a comprehensive, integrated diagnostic cytogenetic and molecular genetic testing service
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CONTACT US(04) 918-5352wrgl@ccdhb.org.nz Please note that genetic test results are only released directly to the referring clinician and not to patients |
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